Lot or serial number not available to pick
The lot written on the warehouse line has no free quantity left: it is reserved for another document, already committed to a second open activity, or no longer on hand in the quantity requested. Business Central checks availability again when you register, not only when the pick or put-away was created, which is why the line looked fine earlier. Find what holds the quantity, release it or point the line at a lot that is genuinely available, then register again.
Tracking numbers are not interchangeable. When a pick, put-away or movement line names Lot No. LOT0001, the system has to find that exact lot free in the bin it wants to take it from, and it verifies this at the moment of registration rather than at creation. Between those two moments another user can reserve the lot for a sales order, a second activity can be created for the same quantity, or the goods can be consumed, adjusted out or moved to another bin. Any of those leaves the line pointing at quantity that is no longer available to it.
Most cases come from a gap between planning and execution: the same stock is committed twice because two documents were prepared at different moments. Registering warehouse activities promptly, setting the reservation policy on the item card to what the warehouse actually follows, and using Select Entries in Item Tracking Lines so only lots on hand can be chosen all reduce the number of lines that go stale. A short daily review of open picks and put-aways also catches activities that were created and then never registered.
No, it is the same check. It reads Serial No. SN00123 is not available on inventory or it has already been reserved for another document, and both the causes and the fix are identical.
Yes. Deleting it releases the lines and returns the quantity to the source document, so you can create the activity again against current availability. Nothing is posted until an activity is registered.